Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 68, Issue 18, Pages 5232-5240Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c01613
Keywords
patulin; fruit-derived food; mycotoxin contamination; biodegradation; ribonucleoside diphosphate reductase; Enterobacter cloacae
Funding
- National Natural Science Foundation of China [31930086, 31722043]
- National Key R&D Program of China [2016YFD0400902]
- Youth Innovation Promotion Association, CAS [Y201919]
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Patulin contamination is a worldwide concern due to its significant impact on human health. Several yeast strains have been screened for patulin biodegradation; however, little information is available on bacterial strains and their mechanism of degradation. In the present study, we isolated a bacterial strain TT-09 and identified it as Enterobacter cloacae subsp. dissolvens based on the BioLog system and 16S rDNA phylogenetic analysis. The strain was demonstrated to be able to transform patulin into E-ascladiol. Isobaric tags for relative and absolute quantitation and reverse transcription quantitative polymerase chain reaction analyses provided evidence that ribonucleoside diphosphate reductase (NrdA), an important enzyme involved in DNA biosynthesis, plays a crucial role in patulin degradation. Deletion of nrdA resulted in a total loss in the ability to degrade patulin in TT-09. These results indicate a new function for NrdA in mycotoxin biodegradation. The present study provides evidence for understanding a new mechanism of patulin degradation and information that can be used to develop new approaches for managing patulin contamination.
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