Flavobacterium supellecticarium sp. nov., isolated from an abandoned construction timber

A bacterial strain CC-CTC003(T) was isolated from a synthetic wooden board. Cells of strain CC-CTC003(T) were Gram-stain -negative, rod-shaped, motile by gliding and formed yellow colonies. Optimal growth occurred at 25 degrees C, pH 7 and in the pres-ence of 1 % NaCl. The phylogenetic analyses based on 16S rRNA genes revealed that strain CC-CTC003(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium cerinum (95.3 % sequence identity), Flavobacterium maris (94.9% sequence identity), Flavobacterium qiangtangense (94.8 %) and Flavobacterium subsaxonicum (94.7 %) and had less than 94.7 % sequence similarity to other members of the genus. Average nucleotide identity (ANI) values between strain CC-CTC003(T) and the type strains of other closely related species were 70.1-74.1 %. The digital DNA-DNA hybridization (dDDH) with F. cerinum was 19.4 %. Strain CC-CTC003(T) contained C-15:0, iso-C-15:0, iso-C-15:0 3-OH, iso-C-17:0 3-OH, summed feature 3 (C-16:1 co6c / C-16:1 co7c) and summed feature 9 (C-16:0 10-methyl / iso-C-17:1 co9c) as the predominant fatty acids. The polar lipid profile consisted of phos-phatidylethanolamine, four uncharacterized aminophospholipids, two aminolipids and one unidentified glycolipid. The major polyamine was sym-homospermidine and contained MK-6 as major isoprenoid quinone. The DNA G+C content of the genomic DNA was 39.2 mol%. On the basis of the phylogenetic inference and phenotypic data, strain CC-CTC003(T) should be classified as a novel species, for which the name Flavobacterium supellecticarium sp. nov. is proposed. The type strain is CC-CTC003(T) (=BCRC 81146(T)=JCM 32838(T)).