Journal
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume 15, Issue -, Pages 2219-2230Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S244315
Keywords
electrochemical aptasensor; screen-printed carbon electrode; flower-like gold microstructures; label-free detection; serpin A12; diabetes biomarkers
Funding
- Tehran University of Medical Sciences (TUMS) [95-04-3333731]
- National Institute for Medical Research Development (NIMAD) [958928]
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Purpose: In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs. Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K-3 [Fe (CN) (6)]/K-4 [Fe (CN) (6)] solution (redox probe). Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039- 10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively. Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.
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