Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ijms21082864
Keywords
zebrafish embryos; acute toxicity; silver nanoparticles; chorion pore size; lysosomal activity; apoptosis
Funding
- Ministry of Science and Technology, Taiwan [MOST 106-2314-B-006-029-MY3, MOST 108-2638-B-006 -001 -MY2, MOST 108-2314-B-039-061-MY3]
- China Medical University, Taichung, Taiwan [CMU108-N-09]
- Ministry of Labor, Taiwan [Q105-P005]
- Toxic and Chemical Substances Bureau, Environmental Protection Administration, Executive Yuan, Taiwan [107A024, 109A012]
- Environmental Analysis Laboratory, Environmental Protection Administration, Executive Yuan, Taiwan [Q106-P014]
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As the worldwide application of nanomaterials in commercial products increases every year, various nanoparticles from industry might present possible risks to aquatic systems and human health. Presently, there are many unknowns about the toxic effects of nanomaterials, especially because the unique physicochemical properties of nanomaterials affect functional and toxic reactions. In our research, we sought to identify the targets and mechanisms for the deleterious effects of two different sizes (similar to 10 and similar to 50 nm) of amine-modified silver nanoparticles (AgNPs) in a zebrafish embryo model. Fluorescently labeled AgNPs were taken up into embryos via the chorion. The larger-sized AgNPs (LAS) were distributed throughout developing zebrafish tissues to a greater extent than small-sized AgNPs (SAS), which led to an enlarged chorion pore size. Time-course survivorship revealed dose- and particle size-responsive effects, and consequently triggered abnormal phenotypes. LAS exposure led to lysosomal activity changes and higher number of apoptotic cells distributed among the developmental organs of the zebrafish embryo. Overall, AgNPs of similar to 50 nm in diameter exhibited different behavior from the similar to 10-nm-diameter AgNPs. The specific toxic effects caused by these differences in nanoscale particle size may result from the different mechanisms, which remain to be further investigated in a follow-up study.
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