Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms21072315
Keywords
mineralization; polyphenol; composite; protein-polyphenol interaction; gellan gum; enzyme
Funding
- Research Foundation Flanders (FWO)
- Czech Academy of Sciences (CAS)
- Special Research Fund Bijzonder Onzderzoeksfonds (BOF) of Ghent University, Belgium [01IO3618, BAS094-18, BOF14/IOP/00]
- German Research Foundation (DFG)
- Czech Science Foundation [17-00885S]
- Research Foundation Flanders (FWO) [G043219, G0D7115N]
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Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
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