Journal
BIOTECHNOLOGY PROGRESS
Volume 31, Issue 3, Pages 849-852Publisher
WILEY-BLACKWELL
DOI: 10.1002/btpr.2087
Keywords
ImageJ; image analysis; filamentous microbes; morphology
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The morphological quantification of filamentous microbes represents an important analytical technique in the optimization of bioprocesses involving such organisms, given the demonstrated links between morphology and metabolite yield. However, in many studies, much of this quantification has required some degree of manual intervention, if it has been conducted at all, burdening biotechnologists with a time-consuming process and potentially introducing bias into analyses. Here, software for the automated quantification of filamentous microbes is presented, implemented as a plug-in for the widely used, freely available image analysis package, ImageJ. The software, together with all related source code, documentation and test data, is freely available to the community via an online repository. (C) 2015 American Institute of Chemical Engineers
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