Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 161, Issue -, Pages 389-397Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.05.235
Keywords
Amylolytic enzyme; Bifidobacterium; Comparative genotnic; High amylose corn starch; Resistant starch-degrading enzyme
Funding
- National Research Foundation of Korea (NRF) - Korean government (MSIT, Ministry of Science and ICT) [2017R1A2B4004218]
- National Research Foundation of Korea [5199990214069, 2017R1A2B4004218] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Resistant starch (RS) is a complex prebiotic carbohydrate beneficial to the human gut. In the present study, four genes encoding for putative amylolytic enzymes, likely to be responsible for RS-degradation, were identified in the genome of Bifidobacterium adolescentis P2P3 by comparative genomic analysis. Our results showed that only three enzymes (RSD1, RSD2, and RSD3) exhibited non-gelatinized high amylose corn starch (HACS)-degrading activity in addition to typical alpha-amylase activity. These three RS-degrading enzymes (RSD) were composed of multiple domains, including signal peptide, catalytic domain, carbohydrate binding domains, and putative cell wall-anchoring domains. Typical catalytic domains were conserved by exhibiting seven typical conserved regions (I-VII) found mostly in alpha-amylases. Analysis of enzymatic activity revealed that RSD2 displayed stronger activity toward HACS-granules than RSD1 and RSD3. Comparative genomics in combination with enzymatic experiments confirmed that RSDs might be the key enzymes used by RS-degrading bifidobacteria to degrade RS in a particular ecological niche, such as the human gut. (C) 2020 Elsevier B.V. All rights reserved.
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