Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 148, Issue -, Pages 715-721Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.01.110
Keywords
Antrodia cinnamomea; Sulfated polysaccharides; Ammonium sulfate; Anti-inflammatory activity
Funding
- Ministry of Science and Technology, Taiwan [MOST108-2320-B-077-005-MY3]
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Antrodia cinnamomea is an important medicinal fungus in Taiwan. This study demonstrates changes of complex sulfated polysaccharides (SPS) by fungus A. cinnamomea after ammonium sulfate-feeding and evaluates its anti-inflammatory activities. The addition of 1 mM ammonium sulfate showed maximal sulfate content of SPS in value of 1.82 mmol/g. Ammonium sulfate changes the physiochemical properties of SPS in that area percentage of SPSs (361 kDa) was increased for 1 mM ammonium sulfate to the value of 26 percentage area. SPS of 1 mM ammonium sulfate-fed A. cinnamomea (AM-SPS) had maximal inhibition of LPS-induced tumor necrosis factor (TNF-alpha) release in RAW264.7 macrophage. I kappa-B degradation induced by LPS in macrophages was reversed by AM-SPS. Suppression of NF-kappa B activation might have been responsible for the anti-inflammatory effects. Meanwhile, the inhibition was also due to suppressing the AKT, and ERK signaling pathway. Our finding suggests that ammonium sulfate is a useful nutrient for production of SPS for neutraceutical and pharmaceutical applications. (C) 2020 Elsevier B.V. All rights reserved.
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