Journal
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
Volume 64, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ifset.2020.102402
Keywords
Pulsed electric field; Free and bound phenolic compounds; brewers' spent grain; Box-Behnken design
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Funding
- Spanish Ministry of Economy and Competitiveness (MINECO) [RYC-2015-18795]
- University of Granada Convocatoria de movilidad internacional de estudiantes de doctorado grant
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Brewers' spent grain (BSG) is the most abundant by-product obtained from beer production. However, it contains some bioactive compounds such as phenolic compounds, therefore, the valorization of BSG is important to recovery these compounds and reused them as functional ingredients in food industry. Therefore, in this work, pulsed electric field (PEF) has been used as extraction pre-treatment. PEF parameters such as electric field strength E (0.5, 1.5, 2.5 kV/cm), frequency (50, 100, 150 Hz) and total time of treatment (5, 10, 15 s) were optimized in order to maximize the content of flavan-3-ols, flavonoids, phenolic acid derivates and total free phenolic compounds. Optimal conditions to the maximum value of total free phenolic compounds were the following: 2.5 kV/cm, 50 Hz and 14.5 s. Concentrations of total free and bound phenolic compounds from BSG under these PEF optimum conditions were 2.7 and 1.7 times, respectively, higher than in case of the extraction without PEF pre-treatment, indicating an improvement in the phenolic recovery with the use of PEF as a pretreatment in brewers spent grain samples.
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