Journal
CERAMICS INTERNATIONAL
Volume 42, Issue 2, Pages 2706-2716Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ceramint.2015.11.001
Keywords
Porous granules; Biphasic calcium phosphates; Ionic substitutions; Drug delivery; Levofloxacin
Categories
Funding
- European Regional Development Fund (FEDER) under the PT2020 Partnership Agreement through the COMPETE
- Portuguese Government through the Portuguese Foundation for Science and Technology (FCT) [UID/CTM/50011/2013]
- Institute for Biomedicine iBiMED [UID/BIM/04501/2013]
- Research Units of the University of Aveiro [CBC PEst-OE/SAU/UI0482/2014]
- FCT [SFRH/BD/78355/2011]
- Fundação para a Ciência e a Tecnologia [PEst-OE/SAU/UI0482/2014, SFRH/BD/78355/2011] Funding Source: FCT
Ask authors/readers for more resources
Multifunctional porous calcium phosphate granules intended as osseous fillers and drug carriers were designed. Strontium- and magnesium-co-substituted biphasic hydroxyapatite (HA)/beta-tricalcium phosphate powders (TCP) with compositions close to the mineral part of human bone [(Ca+Sr+Mg)/P=1.62] were prepared by precipitation, heat treated and deagglomerated, and characterized by XRD and SEM. Highly concentrated aqueous suspensions (up to 60 vol%) were prepared from the powders heat treated at 1000 degrees C. Starch was added as pore forming agent and the suspensions were dripped into a setting sodium alginate solution to obtain spherical granules. Drying and sintering enabled obtaining porous granules that were impregnated with an antibiotic solution (levofloxacin), frozen and then lyophilized. The drug release profile was then assessed in vitro by UV spectrophotometry, with the best release profile being obtained for the Sr-doped granules. The granules' osteocompatibility was evaluated using a pre-osteoblastic cell line. The Sr-doped granules exhibited the highest proliferation yields and efficiency in osteoblastic maturation, including acquisition of a differentiated morphology and high levels of secreted alkaline phosphatase. The microstructural features and the in vitro performance of the granules make them promising multifunctional materials for applications in tissue engineering as antibiotic-loaded bone grafts. (C) 2015 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available