Journal
GENES & DEVELOPMENT
Volume 34, Issue 13-14, Pages 965-972Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.338194.120
Keywords
Drosophila melanogaster; BLID; optogenetics; Dorsal Rel transcription factor; Snail; Twist; morphogen; cis-regulatory module; light-inducible degron
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Funding
- National Institutes of Health [R21HD095639, R35GM118146, R21OD017964]
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Graded transcription factors are pivotal regulators of embryonic patterning, but whether their role changes over time is unclear. A light-regulated protein degradation system was used to assay temporal dependence of the transcription factor Dorsal in dorsal-ventral axis patterning of Drosophila embryos. Surprisingly, the high-threshold target gene snail only requires Dorsal input early but not late when Dorsal levels peak. Instead, late snail expression can be supported by action of the Twist transcription factor, specifically, through one enhancer, sna.distal. This study demonstrates that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets.
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