Journal
BIOTECHNOLOGY LETTERS
Volume 37, Issue 6, Pages 1213-1219Publisher
SPRINGER
DOI: 10.1007/s10529-015-1780-x
Keywords
Biohydrogen; Electrohydrogenesis; Enzymatic saccharification; Microbial electrolytic cell; Pretreatment
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Funding
- Department of Science and Technology, Government of India
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Rice straw was pretreated using a microwave-assisted alkali pretreatment method. Cellulose recovery was approximately 82 %. This material was hydrolysed in an optimized enzymatic saccharification reaction using cellulase from Lysinibacillus sphaericus. This resulted in saccharification of 49 % of cellulosic biomass into glucose. A single chambered microbial electrolytic cell reactor of volume 2l was built using acrylic plastic sheets with graphite sheet as anode and a stainless-steel mesh as cathode. Shewanella putrefaciens was used as exoelectrogen to oxidize rice straw hydrolysate in the reactor for electrohydrogenesis. The maximum H-2 yield obtained was 801 ml H-2 g(-1) COD removal. Coulombic efficiency of 88 %, cathodic H-2 recovery of 58 % and total H-2 recovery of 51 % with an energy efficiency of 74 % were recorded.
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