Journal
FOOD CHEMISTRY
Volume 313, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.126099
Keywords
Hydrolyzable tannins; Hypoglycemic activity; alpha-Glucosidase inhibition; alpha-Amylase inhibition; Intrinsic fluorescence; Inhibition kinetics
Funding
- 'Piano della Ricerca di Ateneo 2016-2018, Linea d'intervento 2' of Universita degli Studi di Catania
- MIUR ITALY PRIN 2017 [2017A95NCJ_003]
- Ministere de l'Enseignement superieur, de la Recherche et de l'Innovation
- Centre National de la Recherche Scientifique (CNRS)
Ask authors/readers for more resources
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia, which can be counteracted by inhibition of alpha-glucosidase and alpha-amylase, both involved in the carbohydrate metabolism. Fourteen C-glucosidic ellagitannins and three galloylated glucoses were studied as potential alpha-glucosidase and alpha-amylase inhibitors. Most of the compounds were found to be moderate inhibitors of alpha-amylase, but potent inhibitors of alpha-glucosidase, showing low-micromolar IC50 values, far lower than that of the antidiabetic drug acarbose. This selectivity can be an advantage for their possible application as functional food ingredients with anti-diabetic properties because strong alpha-amylase inhibition generally causes undesired side effects. The best inhibitors were selected for further studies. Intrinsic fluorescence measurements confirmed their high affinity towards alpha-glucosidase, highlighting a static quenching mechanism. Circular dichroism measurements and kinetics of inhibition indicated that the most active C-glucosidic ellagitannin roburin D (RobD) is a competitive inhibitor, whereas alpha-pentagalloylglucose (alpha-PGG) acts as a mixed-type inhibitor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available