Journal
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
Volume 13, Issue 3, Pages 171-176Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19393210.2020.1742208
Keywords
Auramine O; synthetic food colour; food; spice; solid phase extraction
Funding
- Graduate University of Science and Technology [GUST.STS.DT2017-HH06]
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Auramine O (AO) is a banned food additive and has been classified as an illegal colourant. Therefore, the presence of AO in food should be strictly monitored. In this study, a sensitive UPLC-MS/MS method was applied to monitor AO in 211 food and spice samples. The optimised separation was achieved with a mobile phase consisting of 100 mM ammonium formate at pH 2.9 and acetonitrile, reversed-phase CORTECS T3 column (2.7 mu m, 2.1 x 100 mm) operated at 40oC with a gradient time of 20.0 min (0-95% methanol) at a flow rate of 0.3 mL/min. Limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.1 mu g/kg and 0.5 mu g/kg, respectively. The results showed that 27.0% of samples were contaminated with AO. Considering the common consumption of sour bamboo shout and turmeric powder by so many consumers, AO exposure is significant and should be decreased.
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