Journal
FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 46, Issue 4, Pages 1255-1277Publisher
SPRINGER
DOI: 10.1007/s10695-020-00786-9
Keywords
Pampus argenteus; Gill transcriptome; Salinity stress; Gene expression
Categories
Funding
- National Natural Science Foundation of China [31772869, 31872586] Funding Source: Medline
- Ningbo Agricultural Major Project [2015C110003] Funding Source: Medline
- Zhejiang Major Science Project [2018C02G2070580] Funding Source: Medline
Ask authors/readers for more resources
Salinity is a major ecological factor in the marine environment, and extremely important for the survival, development, and growth of fish. In this study, gill transcriptomes were examined by high-throughput sequencing at three different salinities (12 ppt as low salinity, 22 ppt as control salinity, and 32 ppt as high salinity) in an importantly economical fish silvery pomfret. A total of 187 genes were differentially expressed, including 111 up-regulated and 76 down-regulated transcripts in low-salinity treatment group and 107 genes differentially expressed, including 74 up-regulated and 33 down-regulated transcripts in high-salinity treatment group compared with the control group, respectively. Some pathways including NOD-like receptor signaling pathway, cytokine-cytokine receptor interaction, Toll-like receptor pathway, cardiac muscle contraction, and vascular smooth muscle contraction were significantly enriched. qPCR analysis further confirmed that mRNA expression levels of immune (HSP90A,IL-1 beta,TNF alpha,TLR2,IP-10,MIG,CCL19, andIL-11) and ion transport-related genes (WNK2,NPY2R,CFTR, andSLC4A2) significantly changed under salinity stress. Low salinity stress caused more intensive expression changes of immune-related genes than high salinity. These results imply that salinity stress may affect immune function in addition to regulating osmotic pressure in silvery pomfret.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available