Journal
FEBS LETTERS
Volume 594, Issue 14, Pages 2240-2253Publisher
WILEY
DOI: 10.1002/1873-3468.13808
Keywords
Arf dimer; membrane scission; small GTPase
Funding
- CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior (Capes) [88881.162167/2017-01]
- Alexander von Humboldt Foundation through a Capes-Humboldt Fellowship
- Klaus Tschira Foundation
- German Research Council (Deutsche Forschungsgemeinschaft (DFG) [Wi654/12-1, MA1278/7-1]
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Dimerization of the small GTPase Arf is prerequisite for the scission of COPI-coated transport vesicles. Here, we quantify the monomer/dimer equilibrium of Arf within the membrane and show that after membrane scission, Arf dimers are restricted to donor membranes. By hydrogen exchange mass spectrometry, we define the interface of activated dimeric Arf within its switch II region. Single amino acid exchanges in this region reduce the propensity of Arf to dimerize. We suggest a mechanism of membrane scission by which the dimeric form of Arf is segregated to the donor membrane. Our data are consistent with the previously reported absence of dimerized Arf in COPI vesicles and could explain the presence of one single scar-like noncoated region in each COPI vesicle.
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