Journal
EXPERIMENTAL EYE RESEARCH
Volume 194, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2020.108027
Keywords
Diabetic retinopathy; Ins2(Akita) mouse model; Placental growth factor; Pigment epithelium-derived factor; Retina; Retinal pigment epithelium
Categories
Funding
- Fundacao para a Ciencia e Tecnologia [SFRH/BD/114051/2016]
- Projetos de Investigacao Cientifica e Desenvolvimento Tecnologico (ICDT) [02/SAICT/2017/028121]
- Marie Curie Reintegration Grant [PIRG-GA-2009-249314]
- Fundacao para a Ciencia e Tecnologia/Ministerio da Educacao e Ciencia, through national funds [UID/Multi/04462/2013]
- FEDER under the PT2020 Partnership Agreement
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Diabetic retinopathy (DR) is considered as a diabetes-related complication that can lead to severe visual impairments. By 2030, it is expected that 1 in 5 adults will suffer from the disease. Suitable animal models for chronic DR are essential for a better understanding of the pathophysiology and to further develop new treatments. The Ins2(Akita) mouse is a type 1 diabetes model that shows signs of both early and late stages of DR, including pericyte loss, increased vascular permeability, increased acellular capillaries and neovascularization. To further characterize DR in the Ins2(Akita) mouse model, we have evaluated the protein levels of the angiogenesis inducers vascular endothelial growth factor (VEGF) and placental growth factor (P1GF) and the angiogenesis inhibitor pigment epithelium-derived factor (PEDF). Additionally, we have analyzed the protein expression profile of the glial markers ionized calcium binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) as well as of the chemokine monocyte chemoattractant protein 1 (MCP-1). In this study we demonstrate that, with disease progression, there is the development of an inflammatory response and an unbalanced expression of proand antiangiogenic factors in the neural retina and in the retinal pigment epithelium (RPE) of Ins2(Akita) mice. Therefore, our data provide support for the diabetic retinopathy features detected in the Ins2(Akita) retina, reflecting what is observed in the human pathology.
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