Journal
EUROPEAN RESPIRATORY JOURNAL
Volume 55, Issue 6, Pages -Publisher
EUROPEAN RESPIRATORY SOC JOURNALS LTD
DOI: 10.1183/13993003.01200-2019
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Funding
- Royal College of Surgeons Pump Priming Grant
- Academy of Medical Sciences Starter Grant for Clinical Lecturers
- NIHR GOSH BRC
- Wellcome Trust [WT107963AIA]
- Sir Henry Wellcome Postdoctoral Fellowship from the Wellcome Trust [WT209199/Z/17]
- UK Regenerative Medicine Platform (UKRMP2) Engineered Cell Environment Hub (MRC) [MR/R015635/1]
- Longfonds BREATH lung regeneration consortium
- Roy Castle Lung Cancer Foundation
- Rosetrees Trust
- UCLH Charitable Foundation
- MRC [MR/K017047/1, MR/L002000/1, G1001539, MR/R015635/1, G108/596] Funding Source: UKRI
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Current methods to replace damaged upper airway epithelium with exogenous cells are limited. Existing strategies use grafts that lack mucociliary function, leading to infection and the retention of secretions and keratin debris. Strategies that regenerate airway epithelium with mucociliary function are clearly desirable and would enable new treatments for complex airway disease. Here, we investigated the influence of the extracellular matrix (ECM) on airway epithelial cell adherence, proliferation and mucociliary function in the context of bioengineered mucosal grafts. In vitro, primary human bronchial epithelial cells (HBECs) adhered most readily to collagen IV. Biological, biomimetic and synthetic scaffolds were compared in terms of their ECM protein content and airway epithelial cell adherence. Collagen IV and laminin were preserved on the surface of decellularised dermis and epithelial cell attachment to decellularised dermis was greater than to the biomimetic or synthetic alternatives tested. Blocking epithelial integrin alpha 2 led to decreased adherence to collagen IV and to decellularised dermis scaffolds. At air-liquid interface (ALI), bronchial epithelial cells cultured on decellularised dermis scaffolds formed a differentiated respiratory epithelium with mucociliary function. Using in vivo chick chorioallantoic membrane (CAM), rabbit airway and immunocompromised mouse models, we showed short-term preservation of the cell layer following transplantation. Our results demonstrate the feasibility of generating HBEC grafts on clinically applicable decellularised dermis scaffolds and identify matrix proteins and integrins important for this process. The long-term survivability of pre-differentiated epithelia and the relative merits of this approach against transplanting basal cells should be assessed further in pre-clinical airway transplantation models.
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