Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 38, Issue 4, Pages 1365-1375Publisher
KARGER
DOI: 10.1159/000443080
Keywords
Bauhina championii flavone; Myocardial ischemia/reperfusion injury; Apoptosis; Inflammation; Peroxidation
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Funding
- National Natural Science Foundation of China [81360041]
- Project of Natural Science Foundation of Guangxi, China [GXNSFAA053148]
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Background/Aims: Previous studies have demonstrated that Bauhinia championii flavone (BCF) exhibits anti-oxidative, anti-hypoxic and anti-stress properties. This study was designed to investigate whether BCF has a cardioprotective effect against myocardial ischemia/reperfusion (I/R) injuries in rats and to shed light on its possible mechanism. Methods: The model of I/R was established by ligating the left anterior descending coronary artery for 30 min, then reperfusing for 180 min. Hemodynamic changes were continuously monitored. The content of malondialdehyde (MDA) as well as the lactate dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were assessed. The release of interleukin-6 (IL-6) was measured by enzyme-linked immunosorbent assay (ELISA). Apoptosis of cardiomyocytes was determined by caspase-3 activity and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The expression of TLR4, NF-kappa Bp65. Bcl-2 and Bax were detected by western blotting. Results: Pretreatment with BCE significantly reduced the serum levels of LDH, MDA and IL-6, but increased the activities of SOD and GSH-Px. It also attenuated myocardial infarct size, reduced the apoptosis rate and preserved cardiac function. Furthermore. BCE inhibited caspase-3 activity and the expression of TLR4, phosphorylated NF-KBp65 and Bax, but enhanced the expression of BcI-2. Conclusion: These results provide substantial evidence that BCE exerts a protective effect on myocardial I/R injury, which may be attributed to attenuating lipid peroxidation, the inflammatory response and apoptosis. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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