Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 40, Issue 6, Pages 1354-1366Publisher
KARGER
DOI: 10.1159/000453188
Keywords
Huangqi Decoction; Oxidative stress; Nitric oxide; Vascular dysfunction; Chronic kidney disease
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Funding
- National Natural Science Foundation of China [81473480, 81403235]
- Innovation Program of Talent Project of Putuo District [2014-A-19]
- Key Medical Discipline Project of Shanghai Municipal Health Bureau [ZK2015A18]
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BACKGROUND/AIMS: Endothelial dysfunction is a major factor in the progression of chronic kidney disease, which correlates with oxidative stress and NO deficiency. Huangqi decoction (HQD) is a potential anti-oxidant ingredient in renoprotection. However, the underlying mechanisms remained identified. Therefore, we investigated whether HQD exhibit improvement in endothelial dysfunction in the 5/6 nephrectomy (Nx) rat model. METHODS:Male Wistar rats (180 - 250 g) were divided into sham, Nx and Nx + HQD (0.05, 0.15 and 0.45 g/kg) group, respectively. Renal function and histology were examined with ELISA and Immunohistochemical analysis. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Oxidative stress and NO bioavailability were detected by ELISA, DHE-staining, DAF-2 staining and western blotting. RESULTS: Compared with Nx rats, HQD treatment reversed the functional and structural changes of kidney significantly. Besides, endothelium-dependent relaxation of rat aortas was also improved by HQD treatment. NADPH oxidase and ROS generation were inhibited while NO bioavailability was enhanced. CONCLUSION: HQD can act as a potent prescription for the treatment of endothelium related vascular complications. 2016 The Author(s) Published by S. Karger AG, Basel.
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