Journal
EUROPEAN JOURNAL OF CLINICAL NUTRITION
Volume 74, Issue 9, Pages 1345-1353Publisher
SPRINGERNATURE
DOI: 10.1038/s41430-020-0660-1
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Funding
- Sao Paulo Research Foundation (FAPESP) [2016/06470-7, 2016/05638-1, 2015/186690]
- Centro de Investigacion Biomedica En Red (CIBERobn)
- Instituto de Salud Carlos III (ISCIII), Spain [PI17/01287]
- European Regional Development Fund (FEDER)
- MINECO through the ERDF [MTM2014-52876-R, MTM2017-82724-R]
- Xunta de Galicia (Grupos de Referencia Competitiva) through the ERDF [ED431C-2016-015]
- Xunta de Galicia (Centro Singular de Investigacion de Galicia) through the ERDF [ED431G/01]
- ISCII [CP17/00088]
- European Regional Development Fund
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Background/Objectives We aimed to investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity. Methods Eleven women with morbid obesity (age: 36.9 +/- 10.3 years; BMI: 58.5 +/- 10.5 kg/m(2)) were assessed before and after 6 weeks of a hypocaloric dietary intervention. The participants were compared with women of average weight and the same age (age: 36.9 +/- 11.8 years; BMI: 22.5 +/- 1.6 kg/m(2)). Genome-wide DNA methylation analysis was performed in DNA extracted from peripheral blood leukocytes using the Infinium Human Methylation 450 BeadChip assay. Changes (Delta beta) in the methylation level of each CpGs were calculated. A threshold with a minimum value of 10%, p < 0.001, for the significant CpG sites based on Delta beta and a false discovery rate of Results Dietary intervention changed the methylation levels at 16,064 CpG sites. These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways. However, regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women. Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation. Conclusion A short-term hypocaloric intervention in patients with severe obesity partially restored the obesity-related DNA methylation pattern. Thus, the full change of obesity-related DNA methylation patterns could be proportional to the weight-loss rate in these patients after dietary interventions.
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