Journal
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Volume 38, Issue 5, Pages 1939-1951Publisher
KARGER
DOI: 10.1159/000445555
Keywords
Periplocin; Apoptosis; Gastric cancer cell; EGR1
Categories
Funding
- National Natural Science Foundation of China [81502032, 81173611]
- Hebei Province Science and Technology Plan Project [H2015206376]
- Financial department of Hebei Province [20142065]
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Background/Aims: Periplocin is extracted from the traditional herbal medicine cortex periplocae, which has been reported to suppress the growth of cancer cells. However, little is known about its effect on gastric cancer cells. Methods: Gastric cancer cells were treated with periplocin, and cell viability was assessed using MIS assay. Flow cytometry and TUNEL staining were performed to evaluate apoptosis, and protein expression was examined by western blotting. Microarray analysis was used to screen for changes in related genes. Results: We found that periplocin had an inhibitory effect on gastric cancer cell viability in a dose-dependent manner. Periplocin inhibited cell viability via the ERK1/2-EGR1 pathway to induce apoptosis. Periplocin also inhibited the growth of tumor xenografts and induced apoptosis in vivo. Conclusion: Our results show that periplocin inhibits the proliferation of gastric cancer cells and induces apoptosis in vitro and in vivo, indicating its potential to be used as an antitumor drug. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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