Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 27, Issue 15, Pages 17770-17778Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-020-07968-1
Keywords
Pardosa pseudoannulata; Palpal organ; Cadmium; Cadherin; Transcriptome
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Funding
- National Natural Science Foundation of China [Nos.31272339, Nos. 31472017] Funding Source: Medline
- Scientific Research Foundation of Hunan Provincial Education Department [18A024] Funding Source: Medline
- Project of Science and Technology Department of Hunan Province [No.2014FJ2003] Funding Source: Medline
- Tropical Disease Control and Research, Ministry of Education in China [2018kfkt03] Funding Source: Medline
- Education and Scientific Research Project of Shanghai (CN) [16C0778] Funding Source: Medline
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Pardosa pseudoannulata (Araneae: Lycosidae), as an important predator of crop pests, has served as a strong driver for ecological regulation of pests. Cadmium (Cd) is a toxic heavy metal widely distributed in the soil in China, which not only seriously pollutes the ecological environment, but also poses a great threat to the survival of organisms. Palpal bulbs are the genital organs of male spiders, playing an important role in reproductive physiology. However, the effects of long-term Cd stress on the genital organ of the primary pest predator were poorly understood. Therefore, we investigated the Cd effect on the male palpal organ of P. pseudoannulata at morphological and gene expression levels. The results showed that no obvious difference in the morphology between the Cd-treated and control groups was observed, but cell adhesion was affected at molecular level. Transcriptome sequencing analysis revealed that under long-term Cd stress, the biological processes including cell-cell adhesion via plasma-membrane adhesion molecules, cell-cell adhesion, and homophilic cell adhesion via plasma membrane adhesion molecules were the top three differentially expressed terms (p-adj < 0.001), and 51 unigenes were annotated into cadherin-related proteins, such as protocadherin, cadherin-87A, and cadherin-96Ca, among which, 18 unigenes were significantly upregulated under the Cd stress. Our outcomes indicate that the differentially expressed genes involved in cell adhesion may explain the negative effects of Cd stress on the spider genital organ, and the comprehensive transcriptome dataset will also provide a profound molecular information of the genital organ of P. pseudoannulata.
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