Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 54, Issue 9, Pages 5864-5873Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.9b07651
Keywords
-
Categories
Funding
- National Centre for Research and Development (Poland) under the LIDER V programme [LIDER/013/261/L-5/13/NCBR/2014]
- National Science Centre, Poland [2017/26/E/ST8/00007]
Ask authors/readers for more resources
Chain elongation is a process that produces medium chain fatty acids such as caproic acid, which is one of the promising products of the carboxylate platform. This study analyzed the impact of bioaugmentation of heat-treated anaerobic digester sludge with Clostridium kluyveri (AS + Ck) on caproic acid production from a mixed substrate (lactose, lactate, acetate, and ethanol). It was compared with processes initiated with non-augmented heat-treated anaerobic digester sludge (AS) and monoculture of C. kluyveri (Ck). Moreover, stability of the chain elongation process was evaluated by performing repeated batch experiments. All bacterial cultures demonstrated efficient caproate production in the first batch cycle. After 18 days, caproate concentration reached 9.06 +/- 0.43, 7.86 +/- 0.38, and 7.67 +/- 0.37 g/L for AS, Ck, and AS + Ck cultures, respectively. In the second cycle, AS microbiome was enriched toward caproate production and showed the highest caproate concentration of 11.44 +/- 0.47 g/L. On the other hand, bioaugmented culture showed the lowest caproate production in the second cycle (4.10 +/- 0.30 g/L). Microbiome analysis in both AS and AS + Ck culture samples indicated strong enrichment toward the anaerobic order of Clostridia. Strains belonging to genera Sporanaerobacter, Paraclostridium, Haloimpatiens, Clostridium, and Bacillus were dominating in the bioreactors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available