Journal
EMBO JOURNAL
Volume 39, Issue 10, Pages -Publisher
WILEY
DOI: 10.15252/embj.2019103111
Keywords
autophagy; Beclin 1; CHK2; oxidative stress; ROS
Categories
Funding
- National Key RAMP
- D Program of China [2016YFC1302400]
- Natural Science Foundation of China [81502400, 81702738, 31300963]
- Ministry of Education Innovation Team Development Plan [IRT13101/17R107]
- Natural Science Foundation of Liaoning Province of China [L201571]
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The homeostatic link between oxidative stress and autophagy plays an important role in cellular responses to a wide variety of physiological and pathological conditions. However, the regulatory pathway and outcomes remain incompletely understood. Here, we show that reactive oxygen species (ROS) function as signaling molecules that regulate autophagy through ataxia-telangiectasia mutated (ATM) and cell cycle checkpoint kinase 2 (CHK2), a DNA damage response (DDR) pathway activated during metabolic and hypoxic stress. We report that CHK2 binds to and phosphorylates Beclin 1 at Ser90/Ser93, thereby impairing Beclin 1-Bcl-2 autophagy-regulatory complex formation in a ROS-dependent fashion. We further demonstrate that CHK2-mediated autophagy has an unexpected role in reducing ROS levels via the removal of damaged mitochondria, which is required for cell survival under stress conditions. Finally, CHK2(-/-) mice display aggravated infarct phenotypes and reduced Beclin 1 p-Ser90/Ser93 in a cerebral stroke model, suggesting an in vivo role of CHK2-induced autophagy in cell survival. Taken together, these results indicate that the ROS-ATM-CHK2-Beclin 1-autophagy axis serves as a physiological adaptation pathway that protects cells exposed to pathological conditions from stress-induced tissue damage.
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