Journal
CELL TRANSPLANTATION
Volume 25, Issue 10, Pages 1787-1800Publisher
SAGE PUBLICATIONS INC
DOI: 10.3727/096368916X691303
Keywords
Mesenchymal stem cells (MSCs); Magnetic resonance imaging (MRI); Cell tracking; Superparamagnetic nanoparticles; Intraparenchymal administration
Funding
- Institute de Salud Carlos III [PI13/00292, PI14/01879]
- Spanish Research Network on Cerebrovascular Diseases RETICS-INVICTUS [RD12/0014]
- Xunta de Galicia (Conselleria Education) [GRC2014/027]
- European Union program FEDER
- POCTEP (Operational Programme for Cross-border Cooperation Spain-Portugal) program [0681_INVENNTA_1_E]
- ERDF (European Regional Development Fund)
- Miguel Servet Program of Institute de Salud Carlos III [CP12/03121, CP14/00154]
- Mineco [MAT2013-40971-R]
- Xunta de Galicia [EM2013-046]
- IWT-Vlaanderen
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Superparamagnetic iron oxide nanoparticles (MNPs) together with magnetic resonance imaging (MRI) are the preferred tools for monitoring the fate and biodistribution of administered cells in stem cell therapy studies. Commercial MNPs need transfection agents and long incubation times for sufficient cell labeling and further in vivo cell detection. In this work, we have synthesized MNPs coated with pluronic F127 and tetronic 908, and validated their applicability as contrast agents for MRI cell detection on two different cell types: rat mesenchymal stem cells (MSCs) and multipotent neural progenitor cell line from mice (C17.2). No transfection agent was needed for a complete MNP internalization, and the uptake was only dependent on MNP concentration in medium and limited on the incubation time. By combining in vivo MRI and ex vivo histology microscopy, we have demonstrated the MRI signal detected corresponded exclusively to labeled cells and not to free particles. Pluronic F127- and tetronic 908-coated MNPs represent promising contrast agents for stem cell tracking due to their ease of use in preparation, their efficiency for cell labeling, and their high sensitivity for in vivo cell detection.
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