Journal
CELL STEM CELL
Volume 18, Issue 1, Pages 53-65Publisher
CELL PRESS
DOI: 10.1016/j.stem.2015.12.002
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Funding
- MGH Collaborative Center for X-linked Dystonia-Parkinsonism
- NIH [U01HL100408, U01HL107440, R01DK095384, R01DK097768]
- Harvard Stem Cell Institute
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Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.
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