Journal
CYTOMETRY PART A
Volume 97, Issue 10, Pages 1019-1023Publisher
WILEY
DOI: 10.1002/cyto.a.24031
Keywords
flow cytometry; human PBMC; gamma delta T cells; NK cells; T cells; MAIT cells; innate lymphoid cells
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Funding
- Center for AIDS Research, University of Washington [AI027757]
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases [5U19AI128914-04, UM1 AI068618]
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This 27-color flow cytometry panel was developed in order to assess immunological changes over the course of an immunization and challenge regimen in two experimental malaria vaccine trials. The aim of the study was to find correlates of vaccine-induced protection. Several studies have indicated that protection against malaria appears to involve immune responses at various immunological sites, with liver-resident responses playing an essential role. As it is not feasible to monitor the immune responses within the liver in humans, this panel is developed with the aim to thoroughly characterize the immune responses over time in blood in addition to detecting changes that might reflect what happens in other immunological sites like the liver. The focus of this panel is to detect several innate lymphoid cell populations, including NK cells and their activation status. Moreover, unconventional T cells like mucosal associated invariant T cells and gamma delta T cells are assessed in the panel. (c) 2020 International Society for Advancement of Cytometry
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