Journal
BIOTECHNOLOGY FOR BIOFUELS
Volume 8, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/s13068-015-0286-z
Keywords
Yarrowia lipolytica; Oleaginous yeast; Biolipid production; Crude glycerol; Molasses; Continuous culture; Triglyceride
Funding
- French National Institute for Agricultural Research (INRA)
- INRA
- French National Research Agency (Investissements d'avenir program) [ANR-11-BTBR-0003]
- European Union in the form of an AgreenSkills Fellowship (Marie-Curie FP7 COFUND People Program) [267196]
- Agence Nationale de la Recherche (ANR) [ANR-11-BTBR-0003] Funding Source: Agence Nationale de la Recherche (ANR)
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Background: Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. Results: In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h(-1); 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y-L/gly) and the coefficient of lipid yield to biomass yield (Y-L/X) were equal to 0.1 and 0.4, respectively. Conclusions: These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced.
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