Journal
BIOTECHNOLOGY FOR BIOFUELS
Volume 8, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13068-015-0337-5
Keywords
Photosynthesis; Nannochloropsis; Mutants; EMS; Insertional mutagenesis; Biodiesel; Algae
Funding
- ERC starting Grant BIOLEAP [309485]
- PRIN [2012XSAWYM]
- Centro studi di economia e tecnica dell'energia Giorgio Levi Cases of the University of Padova
- European Research Council (ERC) [309485] Funding Source: European Research Council (ERC)
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Background: The productivity of an algal culture depends on how efficiently it converts sunlight into biomass and lipids. Wild-type algae in their natural environment evolved to compete for light energy and maximize individual cell growth; however, in a photobioreactor, global productivity should be maximized. Improving light use efficiency is one of the primary aims of algae biotechnological research, and genetic engineering can play a major role in attaining this goal. Results: In this work, we generated a collection of Nannochloropsis gaditana mutant strains and screened them for alterations in the photosynthetic apparatus. The selected mutant strains exhibited diverse phenotypes, some of which are potentially beneficial under the specific artificial conditions of a photobioreactor. Particular attention was given to strains showing reduced cellular pigment contents, and further characterization revealed that some of the selected strains exhibited improved photosynthetic activity; in at least one case, this trait corresponded to improved biomass productivity in lab-scale cultures. Conclusions: This work demonstrates that genetic modification of N. gaditana has the potential to generate strains with improved biomass productivity when cultivated under the artificial conditions of a photobioreactor.
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