Journal
CELL CYCLE
Volume 15, Issue 16, Pages 2108-2114Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2016.1203491
Keywords
development; ES cells; EHT (endothelial to hematopoietic transition); GFI1; GFI1B; hematopoiesis; HSCs (Hematopoietic stem cells); hemogenic endothelium; RUNX1; reprogramming
Categories
Funding
- Cancer Research UK
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Bloodwise
- BBSRC [BB/I001794/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I001794/1] Funding Source: researchfish
- Cancer Research UK [12486, 19565] Funding Source: researchfish
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The first hematopoietic cells are generated very early in ontogeny to support the growth of the embryo and to provide the foundation to the adult hematopoietic system. There is a considerable therapeutic interest in understanding how these first blood cells are generated in order to try to reproduce this process in vitro. This would allow generating blood products, or hematopoietic cell populations from embryonic stem (ES) cells, induced pluripotent stem cells or through directed reprogramming. Recent studies have clearly established that the first hematopoietic cells originate from a hemogenic endothelium (HE) through an endothelial to hematopoietic transition (EHT). The molecular mechanisms underlining this transition remain largely unknown with the exception that the transcription factor RUNX1 is critical for this process. In this Extra Views report, we discuss our recent studies demonstrating that the transcriptional repressors GFI1 and GFI1B have a critical role in the EHT. We established that these RUNX1 transcriptional targets are actively implicated in the downregulation of the endothelial program and the loss of endothelial identity during the formation of the first blood cells. In addition, our results suggest that GFI1 expression provides an ideal novel marker to identify, isolate and study the HE cell population.
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