Journal
CELL BIOCHEMISTRY AND FUNCTION
Volume 34, Issue 2, Pages 82-94Publisher
WILEY
DOI: 10.1002/cbf.3167
Keywords
vitamin D; photoreceptor cell death; oxidative stress; cytokines; cone cells; retina
Categories
Funding
- Ministry of Health in Saudi Arabia
- Royal Society of London
- TENOVUS Scotland
- National Eye Research Centre
- Rosetrees Trust
- Fight for Sight
- Visual Research Trust
- W.H. Ross Foundation
- Yorkhill Children's Charity
- Fight for Sight [URP12, 1419/20] Funding Source: researchfish
- Glasgow Children's Hospital Charity [YRSS/PSG/2014/06] Funding Source: researchfish
- Rosetrees Trust [M160-F1] Funding Source: researchfish
Ask authors/readers for more resources
Photoreceptor degeneration (PD) refers to a group of heterogeneous outer retinal dystrophies characterized by the death of photoreceptors. Both oxidative stress and inflammation are involved in the pathogenesis of PD. We investigate whether vitamin D has a potential for the treatment of PD by evaluating the anti-oxidative stress and anti-inflammatory properties of the active form of vitamin D-3, 1,, 25-dihydroxyvitamin D-3, in a mouse cone cell line, 661W. Mouse cone cells were treated with H2O2 or a mixture of H2O2 and vitamin D; cell viability was determined. The production of reactive oxygen species (ROS) in treated and untreated cells was measured. The expression of key anti-oxidative stress and inflammatory genes in treated and untreated cells was determined. Treatment with vitamin D significantly increased cell viability and decreased ROS production in 661W cells under oxidative stress induced by H2O2. H2O2 treatment in 661W cells can significantly down-regulate the expression of antioxidant genes and up-regulate the expression of neurotoxic cytokines. Vitamin D treatment significantly reversed these effects and restored the expression of antioxidant genes. Vitamin D treatment also can block H2O2 induced oxidative damages. The data suggested that vitamin D may offer a therapeutic potential for patients with PD.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available