4.7 Article

Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification

Journal

CHINESE CHEMICAL LETTERS
Volume 32, Issue 2, Pages 775-778

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2020.05.041

Keywords

PEC; CdS QDs; Methylene blue; Multiple microRNAs; Strand displaced amplification; Simultaneous biosensing

Funding

  1. National Natural Science Foundation of China [21605093, 21775082]
  2. Shandong Province Higher Educational Program for Young Innovation Talents
  3. Special Foundation for Distinguished Taishan Scholar of Shandong Province [ts201511052]
  4. Major Program of Shandong Province Natural Science Foundation [ZR2018ZC0127]

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A novel PEC biosensor was proposed for highly sensitive and simultaneous detection of microRNA-21 and let-7a based on SDA reaction, achieving detection limits of 6.6 fmol/L and 15.4 fmol/L, respectively. The biosensor showed acceptable results in the simultaneous analysis of miRNA-21 and let-7a in breast cancer patient's serum, suggesting potential value in the diagnosis of miRNA-related diseases.
Herein, we propose a novel photoelectrochemical (PEC) biosensor for dual microRNAs (miRNAs) highly sensitive and simultaneous biosensing based on strand displaced amplification (SDA) reaction. The recognition of HmiR-21 and Hlet-7a by microRNA-21 and let-7a leads to their change in hairpin structures, subsequently initiating the immobilization of abundant CdS quantum dots (CdS QDs) and methylene blue (MB) based on SDA reaction. The immobilized CdS QDs and MB produce both high PEC currents under 430 nm light and 627 nm light illumination, respectively, and the generated PEC currents are closely relied on target miRNAs amounts. Thus, highly sensitive and simultaneous detection of microRNA-21 and let-7a was readily achieved with detection limit at 6.6 fmol/L and 15.4 fmol/L based on 3 sigma, respectively. Further, this PEC biosensor was applied in simultaneous analysis of miRNA-21 and let-7a in breast cancer patient's serum with acceptable results. We expect this biosensor will find more useful application in diagnosis of miRNA-related diseases. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

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