Developing a cadmium resistant rice genotype with OsHIPP29 locus for limiting cadmium accumulation in the paddy crop

Widespread contamination of agricultural soil with toxic metals such as cadmium (Cd) is a major threat to crop production and human health. Metallochaperones are a unique class of proteins that play pivotal roles in detoxifying metallic ions inside cells. In this study, we investigated the biological function of an uncharacterized metallochaperone termed OsHIPP29 in rice plants and showed that OsHIPP29 resides in the plasma membrane and nucleus and detoxifies excess Cd and Zn. OsHIPP29 was primarily expressed in shoots during the vegetative stage and in leaf sheath and spikelet at the flowering stage. It can be differentially induced by excess Cd, Zn, Cu, Fe and Mn. To identify the function of OsHIPP29 in mediating rice response to Cd stress, we examined a pair of OsHIPP29 mutants, RNAi lines and transgenic rice overexpressing OsHIPP29 (OX) under Cd stress. Both mutant and RNAi lines are sensitive to Cd in growth as reflected in decreased plant height and dry biomass. In contrast, the OX lines showed better growth under Cd exposure. Consistent with the phenotype, the OX lines accumulated less Cd in both root and shoot tissues, whereas OsHIPP29 knockout led to higher accumulation of Cd. These results point out that expression of OsHIPP29 is able to contribute to Cd detoxification by reducing Cd accumulation in rice plants. Our work highlights the significance of OsHIPP29-mediated reduced Cd in rice plants, with important implications for further developing genotypes that will minimize Cd accumulation in rice and environmental risks to human health. (C) 2020 Elsevier Ltd. All rights reserved.