Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 26, Issue 57, Pages 12931-12935Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202002178
Keywords
continuous-flow conditions; DNAzymes; metal-organic frameworks; reactivation; stabilization
Categories
Funding
- National Key Research and Development Program of China for International Science & Innovation Cooperation Major Project [2018YFE0113200]
- National Science Foundation of China [21722502]
- Shanghai Rising-Star Program [19QA1403000]
- Shanghai Science and Technology Committee (STCSM) [18490740500]
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DNAzymes are a promising class of bioinspired catalyst; however, their structural instability limits their potential. Herein, a method to stabilize DNAzymes by encapsulating them in a metal-organic framework (MOF) host is reported. This biomimetic mineralization process makes DNAzymes active under a wider range of conditions. The concept is demonstrated by encapsulating hemin-G-quadruplex (Hemin-G4) into zeolitic imidazolate framework-90 (ZIF-90), which indeed increases the DNAzyme's structural stability. The stabilized DNAzymes show activities in the presence of Exonuclease I, organic solvents, or high temperature. Owing to its elevated stability and heterogeneous nature, it is possible to perform catalysis under continuous-flow conditions, and the DNAzyme can be reactivated in situ by introducing K+. Moreover, it is found that the encapsulated DNAzyme maintains its high enantiomer selectivity, demonstrated by the sulfoxidation of thioanisole to (S)-methyl phenyl sulfoxide. This concept of stabilizing DNAzymes expands their potential application in chemical industry.
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