Journal
CHEMISTRY & BIODIVERSITY
Volume 17, Issue 7, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbdv.202000272
Keywords
argonaute proteins; locked nucleic acids; RNA induced silencing complex; pull-down; Western blot; mRNA; RNA recognition
Funding
- Federal State of Hesse (Germany)
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Dysregulation of miRNAs is connected with a multitude of diseases for which antagomirs and miRNA replacement are discussed as therapeutic options. Here, we suggest an alternative concept based on the redirection of RISCs to non-native target sites. Metabolically stable DNA-LNA mixmers are used to mediate the binding of RISCs to mRNAs without any direct base complementarity to the presented guide RNA strand. Physical redirection of a dye-labeled miRNA model and of specific miRNA-programmed RISC fractions present in HeLa extracts is demonstrated by pull-down experiments with biotinylated capture oligonucleotides.
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