Journal
BIOTECHNOLOGY FOR BIOFUELS
Volume 8, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13068-015-0219-x
Keywords
Lipase; P450 fatty acid decarboxylase; Alkenes; Cell-free catalysis; Whole cell catalysis
Funding
- Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists [Y37203410U]
- Applied Basic Research Programs of Science and Technology of Qingdao [14-2-4-10-jch]
- National Natural Science Foundation of China [NSFC 31270855]
- National High Technology Research and Development Program of China [2014AA093510]
- Recruitment Program of Global Experts
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Background: Biogenic hydrocarbons (biohydrocarbons) are broadly accepted to be the ideal 'drop-in' biofuel alternative to petroleum-based fuels due to their highly similar chemical composition and physical characteristics. The biological production of aliphatic hydrocarbons is largely dependent on engineering of the complicated enzymatic network surrounding fatty acid biosynthesis. Result: In this work, we developed a novel system for bioproduction of terminal fatty alkenes (1-alkenes) from renewable and low-cost triacylglycerols (TAGs) based on the lipase hydrolysis coupled to the P450 catalyzed decarboxylation. This artificial biosynthetic pathway was constituted using both cell-free systems including purified enzymes or cell-free extracts, and cell-based systems including mixed resting cells or growing cells. The issues of high cost of fatty acid feedstock and complicated biosynthesis network were addressed by replacement of the de novo biosynthesized fatty acids with the fed cheap TAGs. This recombinant tandem enzymatic pathway consisting of the Thermomyces lanuginosus lipase (Tll) and the P450 fatty acid decarboxylase OleT(JF) resulted in the production of 1-alkenes from purified TAGs or natural oils with 6.7 to 46.0% yields. Conclusion: Since this novel hydrocarbon-producing pathway only requires two catalytically efficient enzymatic steps, it may hold great potential for industrial application by fulfilling the large-scale and cost-effective conversion of renewable TAGs into biohydrocarbons. This work highlights the power of designing and implementing an artificial pathway for production of advanced biofuels.
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