Journal
CHEMICAL BIOLOGY & DRUG DESIGN
Volume 96, Issue 2, Pages 768-772Publisher
WILEY
DOI: 10.1111/cbdd.13682
Keywords
Epothilone B; fermentation conditions; optimization; orthogonal experiment
Funding
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [2015-1098]
- Chongqing Key Research Project of Basic Science & Frontier Technology [cstc2017jcyjBX0012]
- Foundation Project of Chongqing Normal University [14XYY020]
- Chongqing General Research Program of Basic Research and Frontier Technology [cstc2015jcyjA10054]
- Chongqing Normal University Postgraduate's Research and Innovation Project [YKC17004]
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Epothilone is a macrolide secondary metabolite which has the same anticancer effect as paclitaxel. Based on a series of single-factor experiments, four factors, temperature, initial pH, rotation speed, and inoculum quantity, which have the greatest influence on yield, were determined. Four factors were designed and orthogonal experiments were carried out to optimize the fermentation conditions. Finally, the best experimental conditions were obtained as follows: 250 ml flapper triangular flask was used. The yield of epothilone B was 39.76 mg/L at 30celcius, initial pH = 7.4, rotating speed 200 r/min, inoculation amount 10%, liquid loading amount 50 ml/250 ml, fermentation time 6 days and seed age 60 hr.
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