Journal
CHEMCATCHEM
Volume 12, Issue 16, Pages 4009-4013Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.202000512
Keywords
Photobiocatalysis; non-conventional media; carbon nanodots; organic media; hydroxylation
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Funding
- European Union [764920]
- Marie Curie Actions (MSCA) [764920] Funding Source: Marie Curie Actions (MSCA)
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The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase fromAgrocybe aegerita(AaeUPO) can hydroxylate the neat model substrate cyclohexane. H(2)O(2)was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N-CNDs) and UV LED illumination.AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N-CNDs absorption in beads containingAaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.
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