Journal
CHEMBIOCHEM
Volume 21, Issue 14, Pages 1935-1946Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202000037
Keywords
HaloTag; SNAP-tag; bio-orthogonal chemistry; molecular probes; chemical biology
Funding
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- Paul Berg Early Career Professorship
- Lloyd and Dottie Huck Early Career Award
- Sloan Research Fellowship
- Pew Biomedical Scholars Program
- National Institute of Health [R35GM133484]
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Visualizing and manipulating the behavior of proteins is crucial to understanding the physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain this goal. In this review, we discuss advances in probe technology specific for self-labeling protein tags, focusing mainly on the application of HaloTag and SNAP-tag systems. We describe the latest developments in small-molecule probes that enable fluorogenic (no wash) imaging and super-resolution fluorescence microscopy. In addition, we cover several methodologies that enable the perturbation or manipulation of protein behavior and function towards the control of biological pathways. Thus, current technical advances in the HaloTag and SNAP-tag systems means that they are becoming powerful tools to enable the visualization and manipulation of biological processes, providing invaluable scientific insights that are difficult to obtain by traditional methodologies. As the multiplex of self-labeling protein tag systems continues to be developed and expanded, the utility of these protein tags will allow researchers to address previously inaccessible questions at the forefront of biology.
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