Journal
CELL
Volume 164, Issue 4, Pages 695-709Publisher
CELL PRESS
DOI: 10.1016/j.cell.2015.12.036
Keywords
-
Categories
Funding
- EMBO
- Cancer Research Institute
- Philippe Foundation
- Howard Hughes Medical Institute
- Helen and Martin Kimmel Center for Biology and Medicine
- National Institutes of Health [R21AI084633]
- NCRR [S10 RR023704-01A1]
Ask authors/readers for more resources
Whereas human dendritic cells (DCs) are largely resistant to productive infection with HIV-1, they have a unique ability to take up the virus and transmit it efficiently to T lymphocytes through a process of trans-infection or trans-enhancement. To elucidate the molecular and cell biological mechanism for trans-enhancement, we performed an shRNA screen of several hundred genes involved in organelle and membrane trafficking in immature human monocyte-derived dendritic cells (MDDCs). We identified TSPAN7 and DNM2, which control actin nucleation and stabilization, as having important and distinct roles in limiting HIV-1 endocytosis and in maintaining virus particles on dendrites, which is required for efficient transfer to T lymphocytes. Further characterization of this process may provide insights not only into the role of DCs in transmission and dissemination of HIV-1 but also more broadly into mechanisms controlling capture and internalization of pathogens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available