Journal
CELL
Volume 165, Issue 6, Pages 1416-1427Publisher
CELL PRESS
DOI: 10.1016/j.cell.2016.05.046
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Funding
- Ruth L. Kirschstein National Research Service Award [T32CA009673]
- NIH Pathway to Independence Award [K99CA194077]
- Department of Defense Era of Hope Scholar
- Department of Defense Breast Cancer Collaborative Scholars and Innovators Award
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Transfer RNAs (tRNAs) are primarily viewed as static contributors to gene expression. By developing a high-throughput tRNA profiling method, we find that specific tRNAs are upregulated in human breast cancer cells as they gain metastatic activity. Through loss-of-function, gain-of-function, and clinical-association studies, we implicate tRNAGluUUC and tRNAArgCCG as promoters of breast cancer metastasis. Upregulation of these tRNAs enhances stability and ribosome occupancy of transcripts enriched for their cognate codons. Specifically, tRNAGluUUC promotes metastatic progression by directly enhancing EXOSC2 expression and enhancing GRIPAP1-constituting an inducible pathway driven by a tRNA. The cellular proteomic shift toward a prometastatic state mirrors global tRNA shifts, allowing for cell-state and cell-type transgene expression optimization through codon content quantification. TRNA modulation represents a mechanism by which cells achieve altered expression of specific transcripts and proteins. TRNAs are thus dynamic regulators of gene expression and the tRNA codon landscape can causally and specifically impact disease progression.
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