Journal
CELL
Volume 167, Issue 2, Pages 382-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2016.09.012
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Funding
- SJCRH and NCI [P30 CA021765-35]
- US NIH [AI101935, AI124346, AR056296, CA163507]
- American Lebanese Syrian Associated Charities
- JSPS KAKENHI [15H013770, 15H047450, 15H052750, 15K151220]
- R.G. Menzies Early Career Fellowship from National Health and Medical Research Council of Australia
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The inflammasome is an intracellular signaling complex, which on recognition of pathogens and physiological aberration, drives activation of caspase-1, pyroptosis, and the release of the pro-inflammatory cytokines IL-1 beta and IL-18. Bacterial ligands must secure entry into the cytoplasm to activate inflammasomes; however, the mechanisms by which concealed ligands are liberated in the cytoplasm have remained unclear. Here, we showed that the interferon-inducible protein IRGB10 is essential for activation of the DNA-sensing AIM2 inflammasome by Francisella novicida and contributed to the activation of the LPS-sensing caspase-11 and NLRP3 inflammasome by Gram-negative bacteria. IRGB10 directly targeted cytoplasmic bacteria through a mechanism requiring guanylate-binding proteins. Localization of IRGB10 to the bacterial cell membrane compromised bacterial structural integrity and mediated cytosolic release of ligands for recognition by inflammasome sensors. Overall, our results reveal IRGB10 as part of a conserved signaling hub at the interface between cell-autonomous immunity and innate immune sensing pathways.
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