Journal
CELL HOST & MICROBE
Volume 27, Issue 6, Pages 922-+Publisher
CELL PRESS
DOI: 10.1016/j.chom.2020.04.013
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Funding
- SNF [310030_53074, 310030B_173338/1, Sinergia CRSII_154414/1]
- ETH Zurich [ETH-33 12-2]
- Promedica Foundation
- Helmut Horten Foundation
- Monique Dornonville de la Cour Stiftung
- Deutsche Forschungsgemeinschaft [LA 4572/1-1]
- Swiss National Science Foundation [31003A-173094, 310030_184664, CRSII5_177164]
- ETH
- NIH [R03 AI139557, R01 AI136520, R01 AI044198, USDA 2017-67015-26085, 2017-67017-26180]
- Swiss National Science Foundation (SNF) [CRSII5_177164, 310030_184664] Funding Source: Swiss National Science Foundation (SNF)
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Initial enteropathogen growth in the microbiota-colonized gut is poorly understood. Salmonella Typhimurium is metabolically adaptable and can harvest energy by anaerobic respiration using microbiota-derived hydrogen (H-2) as an electron donor and fumarate as an electron acceptor. As fumarate is scarce in the gut, the source of this electron acceptor is unclear. Here, transposon sequencing analysis along the colonization trajectory of S. Typhimurium implicates the C4-dicarboxylate antiporter DcuABC in early murine gut colonization. In competitive colonization assays, DcuABC and enzymes that convert the C4-dicarboxylates aspartate and malate into fumarate (AspA, FumABC), are required for fumarate/H-2-dependent initial growth. Thus, S. Typhimurium obtains fumarate by DcuABC-mediated import and conversion of L-malate and L-aspartate. Fumarate reduction yields succinate, which is exported by DcuABC in exchange for L-aspartate and L-malate. This cycle allows S. Typhimurium to harvest energy by H-2/fumarate respiration in the microbiota-colonized gut. This strategy may also be relevant for commensal E. coli diminishing the S. Typhimurium infection.
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