Journal
CELL
Volume 165, Issue 7, Pages 1789-1802Publisher
CELL PRESS
DOI: 10.1016/j.cell.2016.05.007
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Funding
- Shelby White and Leon Levy fellowship
- Rockefeller University Women & Science Initiative
- Human Frontier Long-Term Fellowship
- EMBO Long-Term Fellowship
- Sir Henry Wellcome Fellowship
- Eunice Kennedy Shriver National Institute of Child Health and Development of the NIH [F32HD078040]
- NARSAD Young Investigator award
- NIH [1R01HD082131-01A1]
- Rockefeller University
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Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to un-cover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.
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