Journal
CELL
Volume 166, Issue 2, Pages 380-393Publisher
CELL PRESS
DOI: 10.1016/j.cell.2016.06.014
Keywords
-
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [HU363/10-5, HU363/12-1, GraKo1721, SFB646]
- Kekule Fellowship (VCI)
Ask authors/readers for more resources
The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of similar to 70 assembly factors and several small nucleolar RNAs ( snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large a-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available