Journal
CARBOHYDRATE POLYMERS
Volume 236, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116042
Keywords
Collagen; Hyaluronan; Enzymatic cross-linking; Composite hydrogels; Fibrillogenesis
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Funding
- L'Agence Nationale de la Recherche (ANR) [ANR-19-CE06-0028]
- Swiss National Science Foundation (SNSF): INDEED project [310030E_189310]
- Swiss National Science Foundation (SNF) [310030E_189310] Funding Source: Swiss National Science Foundation (SNF)
- Agence Nationale de la Recherche (ANR) [ANR-19-CE06-0028] Funding Source: Agence Nationale de la Recherche (ANR)
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A platform of enzymatically-crosslinked Collagen/Tyramine hyaluronan derivative (Col/HA-Tyr) hydrogels with tunable compositions and gelation conditions was developed to evaluate the impact of the preparation conditions on their physical, chemical and biological properties. At low HA-Tyr content, hydrogels exhibited a fibrillar structure, with lower mechanical properties compared to pure Col hydrogels. At high HA-Tyr and Horse Radish Peroxydase (HRP) content, a microfibrillar network was formed beside the banded Col fibrils and a synergistic effect of the hybrid structure on mechanical properties was observed. These hydrogels were highly resistant against enzymatic degradation while keeping a high degree of hydration. Unlike HA-Tyr hydrogels, encapsulation of human dermal fibroblasts within Col/HA-Tyr hydrogels allowed for high cell viability. These results showed that high HA-Tyr and HRP concentrations are required to positively impact the physical properties of hydrogels while preserving collagen fibrils. Those Col/HA-Tyr hydrogels appear promising for novel tissue engineering applications following a biomimetic approach.
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