Journal
CARBOHYDRATE POLYMERS
Volume 233, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.115832
Keywords
Controlled release; Alginate; Temperature-responsive; BMP-2; Biomineralization; Bone repair
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Funding
- National Research Foundation of Korea - Korean Government (MEST) [20100027955]
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2017R1D1A1B03028061]
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In the present study, a type of bioconjugate was synthesized by post modification of alginate by conjugating temperature-responsive poly(epsilon-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(e-caprolactone-co-lactide) and O-phosphorylethanolamine as phosphorylation functional groups. Freely flowing bioconjugate sols at low temperature can transform to stable viscoelastic gels at the physiological temperature (37 degrees C). Subcutaneous administration of temperature-responsive bioconjugate sols into the dorsal region of Sprague-Dawley rats formed in situ hydrogel. in situ formation of bioconjugate gels in stimulated body fluids at 37 degrees C showed nucleation and hydroxyapatite mineral growth. Furthermore, hydroxyapatite growth was also found in in vivo gels, which suggested the potential of alginate-based bioconjugate gels as a scaffold for bone engineering. Bone morphogenetic protein 2 (BMP-2)-loaded bioconjugate formed stable gel in vivo, and demonstrated sustained release. BMP-2-loaded bioconjugates exhibited in situ biomineralization in vivo. These results imply that the in situ formation of injectable biomimetic materials has potential for bone tissue engineering applications.
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