Journal
BRIEFINGS IN BIOINFORMATICS
Volume 22, Issue 3, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bib/bbaa089
Keywords
microRNA activation; pan-cancer; correlation; genetic mechanism; epigenetic regulation
Funding
- National Institute of Health [GM123037, U01AR0 69395]
- NIH [R01CA172115, R01CA131231]
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It has been found that miRNAs can activate and suppress gene expression, with different interaction patterns in normal and cancer conditions. The miRactDB database provides comprehensive annotation of miRNA-gene interactions, revealing the biological implications of these interactions across various tissue types in cancer and healthy scenarios.
It has been increasingly accepted that microRNA (miRNA) can both activate and suppress gene expression, directly or indirectly, under particular circumstances. Yet, a systematic study on the switch in their interaction pattern between activation and suppression and between normal and cancer conditions based on multi-omics evidences is not available. We built miRactDB, a database for miRNA-gene interaction, at https://ccsm.uth.odu/miRactDB, to provide a versatile resource and platform for annotation and interpretation of miRNA-gene relations. We conducted a comprehensive investigation on miRNA-gene interactions and their biological implications across tissue types in both tumour and normal conditions, based on TCGA, CCLE and GTEx databases. We particularly explored the genetic and epigenetic mechanisms potentially contributing to the positive correlation, including identification of miRNA binding sites in the gene coding sequence (CDS) and promoter regions of partner genes. Integrative analysis based on this resource revealed that top-ranked genes derived from TCGA tumour and adjacent normal samples share an overwhelming part of biological processes, which are quite different than those from CCLE and GTEx. The most active miRNAs predicted to target CDS and promoter regions are largely overlapped. These findings corroborate that adjacent normal tissues might have undergone significant molecular transformations towards oncogenesis before phenotypic and histological change; and there probably exists a small yet critical set of miRNAs that profoundly influence various cancer hallmark processes. miRactDB provides a unique resource for the cancer and genomics communities to screen, prioritize and rationalize their candidates of miRNA-gene interactions, in both normal and cancer scenarios. Significance miRactDB, the first resource for comprehensive annotation of miRNA-gene relation, provides expression profiles, prognostic landscapes and potential mechanisms of action across multiple cancer and healthy tissue types.
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