Journal
BIOSENSORS & BIOELECTRONICS
Volume 154, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112073
Keywords
Extracellular vesicle; Isolation; Butterfly; Microfluidics; Biochip
Categories
Funding
- Strategic Priority Research Program of the Chinese Academy of Science [XDA16010303]
- National Key Research and Development Program of China [2017YFA0700404, 2017YFA0103903]
- NSAF Foundation of China [U1530260]
- Natural Science Foundation of Jiangsu [BE2018707, BE2019711]
- National Natural Science Foundation of China [81622013, 81970882]
- Boehringer Ingelheim Pharma GmbH
- K. C. Wong Education Foundation
- Scientific Research Foundation of Southeast University
- Scientific Research Foundation of the Graduate School of Southeast University
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With the function of mediating intercellular communication between cells, extracellular vesicles (EVs) have been intently studied for their physiopathology and clinical application values. However, efficient EV isolation from biological fluids remains a significant challenge. To address this, this work constructs a new microvortex chip that can isolate EVs efficiently by integrating the lipid nanoprobe modified Morpho Menelaus (M. Menelaus) butterfly wing into microfluidic chip. M. Menelaus wing is well known for its orderly arranged periodic nanostructures and can generate microvortex when liquid passes through it, leading to increased interaction between EVs and M. Menelaus wing. In addition, the nanoprobe containing lipid tails can be inserted into EVs through their lipid bilayer membrane structure. Based on the described properties, high-throughput enrichment of EVs with over 70% isolation efficiency was realized. Moreover, it was demonstrated that the nanoprobe system based on M. Menelaus wing enabled downstream biological analysis of nucleic acids and proteins in EVs. Microvortex chips showed potential application value in efficient EV isolation for biomedical research and cancer diagnosis.
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